Scientific Research


Research area:                                                                                                       GroupPro>>  
       Autophagy is a major intracellular degradation pathway critical for the health maintenance of eukaryotes. As it is mediated by vesicular trafficking, the degradation capacity of autophagy far surpasses that of the proteasomal pathway. It is the only known pathway capable of eliminating entire organelles. The current explosion of autophagy research was ignited in the 90s by pioneering works of Daniel J. Klionsky and Yoshinori Ohsumi. The identification of autophagy-related (ATG) genes in yeast greatly facilitated the subsequent expansion of research into multicellular organisms and the exploration of the physiological functions of autophagy.

       The central step in macroautophagy (the best documented form of autophagy) is the formation of autophagosomes, which are double-membrane vesicles responsible for the sequestration and transport of degradation targets. One main focus of our past and current research is the elucidation of the molecular mechanisms underlying the membrane dynamics during autophagosome formation. In addition, we have a general interest in the study of intracellular membrane trafficking and the application of mathematics in biology.


Publications (Updated 2016.01)

  1. Zhu J, Deng S, Lu P, Bu W, Li T, Yu L*, Xie Z*. The Ccl1-Kin28 kinase complex regulates autophagy under nitrogen starvation. J Cell Sci. 2016 Jan1;129(1):135-44.
  2. Li D, Song JZ, Shan MH, Li SP, Liu W, Li H, Zhu J, Wang Y, Lin J, Xie Z*. A fluorescent tool set for yeast Atg proteins. Autophagy. 2015 Jun 3;11(6):954-60.
  3. Dong C, Lin Z, Diao W, Li D, Chu X, Wang Z, Zhou H, Xie Z, Shen Y, Long J. The Elp2 Subunit Is Essential for Elongator Complex Assembly and Functional Regulation. Structure. 2015 Jun 2;23(6):1078-86.
  4. Li D, Song JZ, Li H, Shan MH, Liang Y, Zhu J, Xie Z*. Storage lipid synthesis is necessary for autophagy induced by nitrogen starvation. FEBS Lett. 2015 Jan 16;589(2):269-76.  
  5. Chen Y, Zhou F, Zou S, Yu S, Li S, Li D, Song J, Li H, He Z, Hu B, Björn LO, Lipatova Z, Liang Y*, Xie Z*, Segev N*. A Vps21 endocytic module regulates autophagy. Mol Biol Cell. 2014 Oct 15; 25(20):3166-77.  
  6. Huang WP, Shintani T, Xie Z*. Assays for autophagy I: the cvt pathway and nonselective autophagy. Methods Mol Biol. 2014; 1163:153-64. (Book Chapter)
  7. Backues SK, Chen D, Ruan J, Xie Z*, Klionsky DJ*. Estimating the size and number of autophagic bodies by electron microscopy. Autophagy. 2014 Jan 1;10(1):155-64.
  8. Zou S,  Chen Y,  Liu Y,  Segev N,  Yu S,  Liu Y,  Min G,  Ye M,  Zeng Y,  Zhu X,  Hong B,  Björn LO,  Liang Y*,  Li S* and Xie Z*. Trs130 participates in autophagy through GTPases Ypt31/32 in Saccharomyces cerevisiae. Traffic. 2013 Feb;14(2):233-46.  
  9. Li H,  Li J,  Li Y,  Singh P,  Cao L,  Xu L,  Li D,  Wang Y,  Xie Z,  Gui Y,  Zheng X. Sonic hedgehog promotes autophagy of vascular smooth muscle cells. Am J Physiol Heart Circ Physiol  2012 Dec;303(11):H1319-31.  
  10. Bartholomew CR,  Suzuki T,  Du Z,  Backues SK,  Jin M,  Lynch-Day MA,  Umekawa M,  Kamath A,  Zhao M,  Xie Z,  Inoki K,  Klionsky DJ. Ume6 transcription factor is part of a signaling cascade that regulates autophagy. Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11206-10.  
  11. Yu ZQ,  Ni T,  Hong B,  Wang HY,  Jiang FJ,  Zou S,  Chen Y,  Zheng XL,  Klionsky DJ,  Liang Y,  Xie Z*. Dual Roles of Atg8−PE Deconjugation by Atg4 in Autophagy. Autophagy. 2012 Jun;8(6):883-92.  
  12. Nair U,  Yen WL,  Mari M,  Cao Y,  Xie Z,  Baba M,  Reggiori F,  Klionsky DJ. A role for Atg8-PE deconjugation in autophagosome biogenesis. Autophagy. 2012 May 1;8(5):780-93.
  13. Yi C,  Ma M,  Ran L,  Zheng J,  Tong J,  Zhu J,  Ma C,  Sun Y,  Zhang S,  Feng W,  Zhu L,  Le Y,  Gong X,  Yan X,  Hong B,  Jiang FJ,  Xie Z,  Miao D,  Deng H,  Yu L. Function and molecular mechanism of acetylation in autophagy regulation. Science. 2012 Apr 27; 336(6080):474-7.  
  14. Yang J,  Zhao Y,  Ma K,  Jiang FJ,  Liao W,  Zhang P,  Zhou J,  Tu B,  Wang L,  Kampinga HH,  Xie Z*,  Zhu WG*. Deficiency of hepatocystin induces autophagy through an mTOR-dependent pathway. Autophagy. 2011 Jul;7(7):748-59.  
  15. Wang HY, Ni T, Xie Z*. Current Knowledge of Autophagy in Yeasts. Chinese Journal of Cell Biology. 2010 Dec, 32(6) 829-39. Invited Review.
  16. Nair U,  Cao Y,  Xie Z,  Klionsky DJ. The roles of the lipid-binding motifs of Atg18 and Atg21 in the cytoplasm to vacuole targeting pathway and autophagy. J Biol Chem. 2010 Apr 9;285(15):11476-88.
  17. Xie Z,  Nair U,  Geng J,  Szefler MB,  Rothman ED,  Klionsky DJ. Indirect estimation of the area density of Atg8 on the phagophore. Autophagy. 2009 Feb;5(2):217-20.  
  18. Xie Z,  Nair U,  Klionsky DJ. Dissecting autophagosome formation: the missing pieces. Autophagy. 2008 Oct 1;4(7):920-2.  
  19. Xie Z,  Nair U,  Klionsky DJ. Atg8 controls phagophore expansion during autophagosome formation. Mol Biol Cell. 2008 Aug;19(8):3290-8.  
  20. Xie Z,  Klionsky DJ. Autophagosome formation: core machinery and adaptations. Nat Cell Biol. 2007 Oct;9(10):1102-9. Review.  

*: Corresponding Author(s)


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